Detection of Brucella melitensis by BACTEC NR660 blood culture system

Assessment of the BACTEC NR660 blood culture system for the detection of bacteremia in young children.

The sensitivity of the BACTEC NR660 blood culture system was assessed by using paired bottles of anaerobic (NR7A) and resin-containing aerobic (NR16A) media and conditions and organisms which simulated those found in pediatric practice. Corresponding media (7D and 16B) of the established BACTEC 460 radiometric method served as controls. The performances of the two systems were similar with 50 i...

Comparison of BACTEC 9240 Peds Plus medium and isolator 1.5 microbial tube for detection of Brucella melitensis from blood cultures.

The sensitivity and time to detection of Brucella melitensis by the BACTEC 9240 and the Isolator blood culture systems were compared in a prospective volume-controlled study. Blood sample aliquots, obtained from children with suspected brucellosis, were inoculated into a BACTEC 9240 Peds Plus bottle and into an Isolator 1.5 Microbial Tube. Overall, 122 pairs of blood samples for culture were ob...

Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR

تشخیص بروسلوز با استفاده از سیستم کشت خون ‌ BACTECو تائید نتایج آن با PCR

Abstract: Brucellosis is one of the most common zoonotic diseases in Iran. Growth of Brucella is slow and blood cultures of these bacteria are time-consuming via classical methods. We try to evaluate BACTEC 9120 system capacity in order to detect of bacteremia due to Brucella spp and to confirm isolated bacteria by PCR. Blood culture sample of 102 suspected patients evaluated by BACTEC 9120 sys...

Specimen volume versus yield in the BACTEC blood culture system.

During a 24-month period, 5,625 blood culture specimens were collected at the Seattle Veterans Administration Medical Center in 20-ml volumes and divided into separate 10-ml aliquots. The two aliquots were processed as duplicate sets (set 1, set 2) by the BACTEC system (Johnston Laboratories, Inc., Towson, Md.). Specimens (5 ml) from each set were inoculated into aerobic (6B) and anaerobic (7C/...